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Plasmid#182321PurposePlasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLyn-μDronpa-TagRFP-T
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CYP450-μDpTT
Plasmid#182325PurposeEndoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertDAKAP-μDronpa-TagRFP-T
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-μDpTT
Plasmid#182322PurposeActin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-μDronpa-TagRFP-T
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
UseTags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP4
Plasmid#178070PurposeInsect cell expression of GCP4DepositorInsertGCP4 (TUBGCP4 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A
Plasmid#163988PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationM1457APromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA13c-RFP
Plasmid#126098PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23102Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-lim-4P::his-24::Cerulean
Plasmid#124336PurposeMulticolor Worms for Neuronal IDDepositorInsertlim-4, Cerulean (lim-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFP-RPB1aAmr
Plasmid#75284PurposeExpresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cellsDepositorInsertPOLR2A (POLR2A Human)
UseTagsEYFPExpressionMammalianMutationN792D alpha-amanitin–resistantPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJ179
Plasmid#85996PurposeOne-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2DepositorInsertsEGFP-NT2_cr2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified mouse U6Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
ErkKTR-irFP
Plasmid#111510PurposeReporter of Erk activityDepositorInsertERK KTR (Mapk1 Mouse)
UseLentiviralTagsiRFPExpressionMutationPromoterSSFVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
UseTagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
p181 pK7-HDAC1 (GFP)
Plasmid#11054DepositorInsertHDAC1 (Hdac1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pRK5 alpha6
Plasmid#16036DepositorInsertalpha6 (ITGA6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuroUThTERT+U3-hTR-500
Plasmid#27665DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cre)
Plasmid#174862PurposeExpresses mouse Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by MmPeg10 UTRs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-FKRP-EF1a-BSD
Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorInsertFukutin-related protein (FKRP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
YE2-BE4max
Plasmid#138156PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE2)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R132E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only