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Plasmid#218731PurposeExpresses C-terminally EGFP-tagged IFT80 in mammalian cellsDepositorInsertIFT80 (IFT80 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR10-DuET
Plasmid#213197PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR10 (CCR10 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_RRP9:1-475
Plasmid#211133PurposeMammalian expression of full-length human RRP9. N-terminal HiBiT tag.DepositorInsertRRP9:M1-S475 (RRP9 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro
Plasmid#211687PurposeU6-sgRNA-EFS-Cas9-P2A-PuroDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-deLACCO1-NGR
Plasmid#207990PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196MPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D193E wgMDH
Plasmid#204269PurposeBacterial expression of D193E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD193EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_023d-sgCiCh2-2
Plasmid#202444PurposeExpression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2DepositorInsertChromosome 2 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003-sgKCMF1
Plasmid#202441PurposeExpression of a guide RNA targeting KCMF1DepositorInsertKCMF1 gRNA (KCMF1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-VP64_Blast
Plasmid#192655Purpose3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance markerDepositorInsertscFv-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NLS-CanlonicSF
Plasmid#188390PurposeTo explore lactate dynamics in intact systemsDepositorInsertNLS-CanlonicSF
UseTagsNLS and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH1002-Tier2(pUC ori)
Plasmid#169600PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-β2AR-μDpTT
Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-μDronpa-TagRFP-T (ADRB2 Human)
UseTagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDronpa-N1-Tau-μDpTT
Plasmid#182323PurposeMicrotubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertTau-μDronpa-TagRFP-T
UseTagsMAP tauExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-μDpTT
Plasmid#182327PurposePKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2B-μDpTT
Plasmid#182328PurposeHistone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertH2B-μDronpa-TagRFP-T (H2BC3 Human)
UseTagsFull-length human histone H2BExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-Dronpa
Plasmid#182330PurposeActin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-Dronpa
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only