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901 pLNCX myr HA Akt1

Plasmid

#9005

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DDX54

Plasmid

#97060

Purpose

Expresses FLAG/HA-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only
GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pBabe zeo HMGA2

Plasmid

#17411

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

HMGA2 (Hmga2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Promoter

Available since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7

Plasmid

#221555

Purpose

Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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1013 pcDNA3 T7 Akt1 T308A S473A

Plasmid

#9030

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 T308A S473A (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

T308A S473A

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23108 and PpsdA110

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts2

Plasmid

#115872

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts3

Plasmid

#115873

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSW648 - pREV>>

Plasmid

#115996

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPc

Plasmid

#87166

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPc

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF1472

Plasmid

#141825

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

YFPn

Expression

Mutation

Promoter

35s

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW646 - pML1>>

Plasmid

#115995

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

Plasmid

#205098

Purpose

Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

Inserts

mNeonGreen
eKaz luciferase
Volvox Channelrhodopsin 1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16–RAR-alpha

Plasmid

#80434

Purpose

Expression of constitutively active human RAR-alpha in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Retinoic acid receptor alpha (RARA Human)

Use

Tags

EGFP and VP16

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-FAM208A ts1

Plasmid

#115871

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge