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pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab8a-DN (T22N)
Plasmid#101049PurposeExpresses HA-tagged human Rab8-DN (T22N) in mammalian cellsDepositorInsertRab8a (RAB8A Human)
UseTagsHAExpressionMammalianMutationT22NPromoterCMVAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Bcr pLEF
Plasmid#38160DepositorInsertBcr (BCR Human)
UseTagsGSTExpressionMammalianMutationPromoterSV40Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hPGK-mCherry-SynaptoZip
Plasmid#177317PurposeLentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseLentiviralTagsMyc and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
LPCX BMP-RIB Q203D
Plasmid#12642DepositorInsertBMP-RIB (Bmpr1b Mouse)
UseRetroviralTagsHAExpressionMammalianMutationQ203D, activating mutationPromoterAvailable sinceOct. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
Plasmid#173868PurposeMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)DepositorInsertmVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-Cas9
Plasmid#120353PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 V5 mDispatched WT 2x HA
Plasmid#126410PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.DepositorInsertm-Dispatched1 (Disp1 Mouse)
UseTagsHAExpressionMammalianMutationV5 iserted between A106 and A107PromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
HMGA2-FLAG
Plasmid#25409DepositorInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseRetroviral; ChipTagsFLAGExpressionMammalianMutationNonePromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluu
Plasmid#106122PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
IL2 F42A pAcGP67a
Plasmid#41807DepositorInsertIL2 (IL2 Human)
UseTags6x HisExpressionInsectMutationPhenylalanine 42 to AlaninePromoterPolyhedronAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6 SgRNA GAPDH
Plasmid#162736PurposeMammalian expressionDepositorInsertSpacer sequence for GAPDH
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1
Plasmid#21605DepositorInsertMCL-1 (MCL1 Human)
UseTagsExpressionMammalianMutationInsert ends with a STOP codon, no V5-His-tag.PromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA5/mCherry-pBirA-HA
Plasmid#154458Purposeexpresses mCherry-pBirA-HA/biochemical assayDepositorInsertmCherry-pBirA-HA
UseTagsExpressionMammalianMutationPromoterCMV-F, T7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-sTagRFP-TUBA1B
Plasmid#207768PurposeDonor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p21-pRc/CMV
Plasmid#20814DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only
