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Plasmid#133442PurposeCovalently circularized nanodisc NW9 for studying membrane proteins and viral entry.DepositorInsertNW9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dronpa-Kras
Plasmid#182329PurposePlasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertDronpa-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-Dronpa
Plasmid#182332PurposeAKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertAKAP79-Dronpa (AKAP5 Human)
UseTagsFull-length AKAP79ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-Mito-DsRed
Plasmid#210328PurposeOverexpression of Mito-DsRedDepositorInsertMito-DsRed
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-ATF6
Plasmid#11975PurposeFor mammalian expression of human ATF6 with a 3x FLAG tagDepositorInsertATF6 (ATF6 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
BSP605
Plasmid#122250Purposeexpression vector for mCerulean under EFT3 promoterDepositorInsertmCerulean
UseTagsExpressionWormMutationPromoterEFT3Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]
Plasmid#168173PurposeExpression of BeCyclOp in BWMs of C. elegansDepositorInsertBeCyclOp (codon optimized for C. elegans)
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLRP3-mNeonGreen
Plasmid#200942PurposeNLRP3 fused with mNeonGreen to indicate its localization changeDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_alphaTubulin_C1
Plasmid#85045PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9
Plasmid#137080PurposeExpression of Himar-dCas9 in bacteriaDepositorInsertHimar-dCas9
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-DEVD-TagRFPT
Plasmid#87704PurposeFluorescent biosensor for super-resolution imaging of caspase activity.DepositorInsertDronpa-DEVD-TagRFPT
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).cpSFGFP.HaloTag
Plasmid#214925PurposeExpresses mitochondrially targeted non-responsive controlDepositorInsert(mito).cpSFGFP.HaloTag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
RHPMP140 (pLifeAct mOrange2)
Plasmid#88840PurposeLocalization by Super-Resolution microscopy of lifeAct fused to mOrange2DepositorInsertLifeAct
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS1-gpa-2P::his-24::Cerulean
Plasmid#124337PurposeMulticolor Worms for Neuronal IDDepositorInsertgpa-2, Cerulean (gpa-2 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 1-154 (XSB382)
Plasmid#78510PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. ColiDepositorInsertGrb2 1-154 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-1/2
Plasmid#91480PurposeProtein expression and purification of human SH3 domain construct GRB2-1/2DepositorInsertGRB2-1/2 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only