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Plasmid#42559DepositorInsertshLacZ
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-mTET1
Plasmid#83569PurposeExpresses TET1 with a mutated catalytic domain in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationcatalytic domain mutant H1672D, D1674APromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRB14
Plasmid#52522Purposeexpresses a myc-tagged version of hCas9 in DrosophilaDepositorInsertS. pyogenes cas9 with humanized codon bias
UseTagsmycExpressionInsectMutationPromotertubulinAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Ufm1 ∆C3
Plasmid#133866PurposeExpression of human UFM1 mutant lacking the last three C-terminal residuesDepositorInsertUbiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-Puro
Plasmid#84540PurposeCRISPR - FLIP targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialMutationPromoterPotefAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP16
Plasmid#158367PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP16 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 NSP4
Plasmid#158355PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP4 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-myc3-CUL4A delta N52
Plasmid#19952DepositorInsertCUL4A (cullin 4A) (CUL4A Human)
UseTagsmyc3ExpressionMammalianMutationMissing 52 residues from N terminal CUL4A.PromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-RNMT
Plasmid#112709PurposeExpresses N-terminally GST-tagged human RNMT in bacterial cellsDepositorInsertRNMT (RNMT Human)
UseTagsGST and thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1F+2
Plasmid#190833PurposeFor mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSJ376
Plasmid#103065PurposeExpresses colicin E1 with R domain deletion and ImmE1DepositorUseTagsExpressionBacterialMutationR (receptor-binding domain) deletion in colicin E…PromoterT7 lac (from pET) and T7 lac (from pET52-b)Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-CAND1
Plasmid#19949DepositorInsertCAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NES
Plasmid#175015PurposeCytosol-targeted TORCAR.DepositorInsertTORCAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS
Plasmid#199352PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP-G3BP2(2)
Plasmid#136061PurposeG3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)DepositorInsertG3BP2 (G3BP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT005
Plasmid#182715PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator regionDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
UseTags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only