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Plasmid#79243PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (R38A); PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAB094d
Plasmid#79244PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (E52A); PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73DGW (F3)
Plasmid#74040Purposelentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationS73DPromoterH1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-TurboID-blast
Plasmid#222599PurposeExpress fussed dCas9-TurboID for proximity labelingDepositorInsertdCas9-TurboID
UseLentiviralTagsExpressionMutationPromoterEF-1αAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX_TRE3G_DD::mCherry
Plasmid#108679PurposeTunable system for controlled gene expression and protein stability in mammalian cellsDepositorInsertDestabilizing Domain (DD) mCherry fusion protein
UseLentiviralTagsDD::mCherry fusion proteinExpressionMammalianMutationPromoterDoxycycline inducibleAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160
Plasmid#100931PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 strainDepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
UseTagsmNeptuneExpressionWormMutationPromoterPtwk-40Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
REEP5-EGFP
Plasmid#210363PurposeExpresses REEP5 fused with EGFP in mammalian cellsDepositorInsertREEP5 (REEP5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorUseTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2-mDux
Plasmid#138320PurposeDox-inducible codon-optimized mouse DuxDepositorInsertDux (Dux Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Lifeact-mCherry
Plasmid#185408PurposeRed fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMVDepositorInsertlifeact
UseIn vitro synthesis of mrnaTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFEB
Plasmid#38119PurposeMammalian expression of Transcription factor EB fused to EGFPDepositorInsertTFEB (TFEB Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only