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Plasmid#168226PurposeExpresses human PFAS-3xFLAG fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2-neo-TET
Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterenhancer from 7SL geneAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA
Plasmid#47457PurposeLITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.DepositorInsertNLS(alpha-imp)-CRY2PHR-NLS-VP64
UseTags2A_GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
UseTagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_deltaPLD
Plasmid#139115Purposeexpress FL hnRNPF without the prion like domain with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationdelta365-415PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe WT Akt2
Plasmid#14551DepositorInsertAkt2 (AKT2 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
Plasmid#62459Purposeexpresses his-tagged C288S human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionMammalianMutationChanged Cysteine 288 of Keap1 to serinePromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag Intersectin Short (human)
Plasmid#47392DepositorInsertIntersectin short (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_ELAVL1
Plasmid#106105PurposeInducible gene expression of EGFP ELAVL1DepositorInsertNP_001410.2 (ELAVL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNAZeo(-)HSC73
Plasmid#86030PurposeExpression of Heat shock chaperone 70 in Mammalian cellsDepositorInsertHeat-shock cognate protein 73 (HSPA8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
B2387_mEGFP-FKBP2x
Plasmid#216216PurposeExpresses mEGFP(A206K) fused to FKBP-FKBP in mammalian cellsDepositorInsertmEGFP-FKBP-FKBP (FKBP1A Human)
UseTagsEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorInsertTmpo (Tmpo Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Cortactin
Plasmid#74476PurposeThis plasmid was constructed to study cortactin interactionsDepositorInsertCortactin (Cttn Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18
Plasmid#68827PurposeExpresses c-myc-tagged human RAD18DepositorInsertc-myc tagged human RAD18 (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ mWnt5a
Plasmid#102860PurposeExpresses mouse Wnt5a in mammalian cells, zebrafish or XenopusDepositorInsertMouse Wnt5a
UseZebrafish expression; xenopus expressionTagsExpressionMammalianMutationPromoterCMV; SP6Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only