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Plasmid#133603PurposePart Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)DepositorInsertdCas9 NLS Stop
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM768
Plasmid#133605PurposePart Plasmid for dCas9 NLS, Part 3b(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM769
Plasmid#133606PurposePart Plasmid for dCas9 NLS, Part 3a(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM755
Plasmid#133599PurposePart Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)DepositorInsertsgERG11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM764
Plasmid#133601PurposePart Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)DepositorInsertsgTEF1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTM-BPL
Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
UseTagsFLAG and Signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCCP-GFP-NLS
Plasmid#173923PurposeFluorescent labeling of the nuclear envelopeDepositorInsertBiotin carboxyl carrier protein, AcGFP1
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV Sbf-Sal linker
Plasmid#50434PurposeCloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC D218A
Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23PI-Sce ESARC
Plasmid#195607PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoterDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationC552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…PromoterT7Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-attB V5 dDispatched delta CS HA
Plasmid#126412PurposeExpression of V5 dDispatched Delta CS HA in Drosophila cells linesDepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationDisp1 Cleavage sitePromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-Btgh-3NLS
Plasmid#194469PurposeEncodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA.008
Plasmid#22841DepositorInsertA DNA (with MCS)
UseCotton vigs vectorTagsExpressionBacterialMutationCoat Protein (AR1 gene) of Cotton Leaf Crumple Vi…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVB1.3
Plasmid#22844DepositorInsertB DNA
UseCotton vigs vectorTagsExpressionBacterialMutation1.3 unit B componet length inserted into pBlueScr…PromoterAvailable sinceMarch 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT4 (MT4 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorInsertmetallothionein 3 (MT3 Human)
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorInsertmetallothionein 3 (MT3 Human)
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only