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Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMAD2L1_HORMA (MAD2L1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS1516
Plasmid#205979PurposeFLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas7 from MWEE01000013, Cas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1 sgTollip
Plasmid#196546PurposeLentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF9-STAT2C
Plasmid#37544DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)
Plasmid#101298PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
5HT6-G-GECO1.0
Plasmid#47499PurposeFluorescent reporter for Ca2+ inside primary ciliaDepositorInsert5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
UseTagsG-GECO1.0ExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCas-CmR(+)
Plasmid#113633PurposeChloramphenicol resistant derivative of pCas plasmidDepositorInsertChloramphenicol resistance gene
UseCRISPRTagsExpressionMutationPromoterPcatAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mRuby2
Plasmid#185008PurposeFor knocking in FKBP F36V P2A mRuby2DepositorInsertFKBP F36V P2A mRuby2
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hDDX41-HA
Plasmid#165469PurposeHuman DDX41 mammalian expression vector with C-terminal HA tag.DepositorInsertDEAD-box helicase 41 (DDX41 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11
Plasmid#207758PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4-BG505.SOSIP-8his
Plasmid#111847PurposeMammalian expression plasmid for soluble BG505 SOSIP.664DepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTags8his purification tag and CD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3A
Plasmid#50857Purposeexpresses constituvely active cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX302-JSP1-V5 puro
Plasmid#87773PurposeExpresses V5 tagged human JSP1 (DUSP22)DepositorInsertJSP1 (DUSP22 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K118-119R
Plasmid#63564PurposeExpression of human H2A with K118R and K119R tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine 118 and Lysine 119 are now ArgininePromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBLO 62.5
Plasmid#123124PurposeHuman expression vector for planctomyces CasX and sgRNADepositorInsertsCasX protein
CasX sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-5U-Venus-PSD-95-3U
Plasmid#102949PurposeExpresses Venus-PSD-95 in mammalian cellsDepositorInsertPSD-95 (Dlg4 Mouse)
UseTagsVenus Fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDE-2Strep
Plasmid#210355PurposeExpresses IDE fused with 2Strep in mammalian cellsDepositorInsertIDE (IDE Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Puro-H2BC11
Plasmid#207761PurposeDonor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 SIRT6_H133Y
Plasmid#53149Purposeexpression vector for catalyitc inactive human Sirtuin6DepositorInsertSirt6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationH133Y, catalytic inactivePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Flag-Daxx-Puro
Plasmid#124898PurposeExpress Flag-Daxx in mamalian cellsDepositorInsertDeath domain-associated protein 6 (DAXX )
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only