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Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_FLAG-HUWE1
Plasmid#187121Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPERK-FLAG-IP
Plasmid#101793PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PERKDepositorInsertPerk (Eif2ak3 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA050
Plasmid#216107PurposegDNA expression for Cas9 with ChenF+E trRNA (guide only)DepositorInsertgDNA expression for Cas9 with ChenF+E trRNA
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_181
Plasmid#216102PurposeCRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)DepositorInsertCas9 [Sp]
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-FOXP3-IRES-Thy1.1
Plasmid#17443DepositorInsertFOXP3 (FOXP3 Human)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT GLI2 delta N
Plasmid#17649DepositorInsertGLI2 (GLI2 Human)
UseTags6x MycExpressionMammalianMutationMissing 328 N-terminal amino acidsPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Cdc42BPB
Plasmid#50759Purposeexpression of EGFP tagged Cdc42BPBDepositorInsertCdc42BPB (CDC42BPB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
AA145
Plasmid#215937PurposeFragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)DepositorHas ServiceCloning Grade DNAInsertU6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1.2-Orai1
Plasmid#73562PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertG-GECO1.2-Orai1 (ORAI1 Human, Synthetic)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorInsertCas5-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Pmel-1_TCRB
Plasmid#175288PurposeExpresses Pmel-1 TCR beta chainDepositorInsertT cell receptor beta chain (Tcrb Mouse)
UseSuitable for ivtTagsExpressionMammalianMutationPromoterCMV and T7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Pmel-1_TCRA
Plasmid#175287PurposeExpresses Pmel-1 TCRA alpha chainDepositorInsertT cell receptor alpha chain (Tcra Mouse)
UseSuitable for ivtTagsExpressionMammalianMutationPromoterCMV and T7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0mut
Plasmid#164607PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mPKR-FLAG-IG
Plasmid#101792PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PKRDepositorInsertPkr (Eif2ak2 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD EGFP
Plasmid#187267PurposeLentiviral expression of shRNA targeting CortactinDepositorInsertshRNA for Cortactin (CTTN Human)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Substance P-pHluorin IRES mRuby3
Plasmid#195699PurposeLentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertTac1 (Tac1 Mouse)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aciā¦PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Usp9x_CD-mCherry
Plasmid#170380PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.DepositorInsertUbiquitin specific protease 9x catalytic domain (Usp9x Mouse)
UseTagsmCherry-3xFlagExpressionMammalianMutationCatalytic domainPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -