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Plasmid#206990PurposeA plasmid for expression of SaCas9-sgRNA targeting donor plasmidsDepositorInsertDonor plasmid-targeting SaCas9-gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS1
Plasmid#196977PurposeExpresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS1 tag
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P5
Plasmid#186350PurposeGateway donor vector with AttP3 AttP5 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Gal4DBD2xHighMag1
Plasmid#182023PurposeDonor plasmid for conversion of Gal4 line into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1
UseDonor plasmidTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_2
Plasmid#127555PurposeDrosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
mKusabira Orange-C1 (mKO)
Plasmid#54834PurposeLocalization: C1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneUseTagsmKusabira OrangeExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion Halo-mAID
Plasmid#112852PurposeGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous proteinDepositorInsertHalo-mAID
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTetO-hOSK-CASH-1
Plasmid#188977PurposeA CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.DepositorInsertDoxycycline inducible reprogramming donor cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
SAC1deltaTMD-FKBP-mCherry
Plasmid#108123PurposeRecruitable cytosolic SAC1DepositorInsertSACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1
Plasmid#108127PurposeRecruitable SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-221-P3-P4
Plasmid#186351PurposeGateway donor vector with AttP3 AttP4 recombination sitesDepositorTypeEmpty backboneUseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Spinophilin
Plasmid#87123PurposeGateway donor vector for SpinophilinDepositorInsertSpinophilin (PPP1R9B Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S
Plasmid#108128PurposeRecruitable inactive SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only