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Plasmid#46526DepositorInsertTxk (Txk Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCRT7 tetR pLtetO MBP-MEK1 XX Amp
Plasmid#53225Purposehuman MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesisDepositorInsertMEK1 (MAP2K1 Human)
UsePhosphoprotein synthesisTagsHIS and MBPExpressionBacterialMutationCodon optimized for E.coli, both codons for Ser21…PromoterpLtetOAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
shTCF7L2 # 2
Plasmid#42558DepositorInsertshTCF7L2 (TCF7L2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK2
Plasmid#172979Purposedoxycycline-inducible expression of TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-NES-YFP-STOP-L2
Plasmid#127359Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
psicheck2 PTEN 3'UTR
Plasmid#50936Purposereporter for PTEN RNAiDepositorInsertPTEN 3'UTR (PTEN Human)
UseRNAiTagsRenilla luciferaseExpressionMammalianMutationPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc human CIN85
Plasmid#47935Purposeexpresses Myc tagged human CIN85 in mammalian cellsDepositorInsertCIN85 (SH3KBP1 Human)
UseTagsMycExpressionMammalianMutationcontains amino acids 2-665 of human CIN85; D114GPromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKMD111e - intein-Cas9(S219-G521R)
Plasmid#64192PurposeExpresses intein-Cas9(S219-G521R) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry
Plasmid#75132PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MTS-KD-c-Src-FLAG
Plasmid#44653DepositorInsertv-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
UseTagsFLAG tag and mitochondria-targeting sequence (MTS…ExpressionMammalianMutationchanged lysine 298 to methioninePromoterCMV promoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track HA PGC-1 alpha
Plasmid#14427DepositorInsertPGC-1 alpha (Ppargc1a Mouse)
UseAdenoviralTagsHAExpressionMammalianMutationLast four amino acids of PGC-1a removed to make t…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBLO ddd64.1
Plasmid#123121PurposeE. coli expression vector for catalytically inactivated deltaproteobacteria CasXDepositorInsertCasX
UseCRISPRTagsExpressionMutationMutated amino acids in catalytic triad to alanine…PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem214
Plasmid#175153PurposeLentiviral expression of V5-tagged mouse Tmem214DepositorInsertTmem214 (Tmem214 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-mem
Plasmid#55622PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.DepositorInsertmRFP-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…PromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-puro-3xFLAG-RUNX3 R122C
Plasmid#203439PurposeMammalian expression of RUNX4DepositorInsertRUNX3 (RUNX3 Human)
UseTags3 x FLAGExpressionMammalianMutationR122CPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQ and SV40Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(1363-1370Mut)-MBP
Plasmid#190038PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and MBPExpressionMammalianMutation1363 RDKDLIES 1370 to GSSGGSSGPromoterCMVAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only