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Plasmid#16155DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS400APromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-sgSORCS2-G2
Plasmid#118416PurposeCRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2DepositorInsertgRNA SORCS2 Human (SORCS2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4DdeltaC
Plasmid#51602Purposeexpresses Sema4D lacking C terminusDepositorInsertSema4DdeltaC (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationLast 69 amino acids of Sema4D C-terminus are dele…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTmut full-length
Plasmid#109162PurposeEncodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationCys402SerPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Barkor 1-91
Plasmid#24395DepositorInsertBarkor (ATG14 Human)
UseTagsHis and MycExpressionMammalianMutationContains amino acids 1-91.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Barkor 1-180
Plasmid#24396DepositorInsertBarkor (ATG14 Human)
UseTagsHis and MycExpressionMammalianMutationContains aa 1-180.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment A
Plasmid#86658PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the NcoI site from 880 (…PromoterCDK6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
tdEos-VASP-5
Plasmid#57685PurposeLocalization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVASP
UseTagstdEosExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEVOL_PylRS(AF)-tRNA
Plasmid#223511PurposePylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsUseTags6xHisExpressionBacterialMutationY306A; Y384FPromoteraraBAD, rrnB, and glnS and proKAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SC_4.26_STARR-seq
Plasmid#220220PurposeAAV-STARR-seq screening vector with 4.26 promoteDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoter4.26Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA
Plasmid#220225PurposeAAV-STARR-seq validation vector containing VS33 enhancerDepositorInsertEnhancer (VS33)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS N terminal HA
Plasmid#217639PurposeEntry vector with WT1 +KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS C terminal HA
Plasmid#217640PurposeEntry vector with WT1 +KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only