pET41sIII_GST-ALC1
(Plasmid
#173932)
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PurposeBacterial expression of ALC1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET41sIII
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameALC1
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SpeciesH. sapiens (human)
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Entrez GeneCHD1L (a.k.a. ALC1, CHDL)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protein residues 1-897 (a start codon included in-frame), backbone is modified version of pET41
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET41sIII_GST-ALC1 was a gift from Joan Conaway (Addgene plasmid # 173932 ; http://n2t.net/addgene:173932 ; RRID:Addgene_173932) -
For your References section:
Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling. Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. 10.1073/pnas.2107277118 PubMed 34465625
