Showing: 8641 - 8660 of 145688 results
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Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT
Plasmid#60814Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFP-NLS (SV40)ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCri-1a
Plasmid#60816PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and MBP-tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV5-25BoxB
Plasmid#60817PurposeCloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFPDepositorInsert25x BoxB
UseSynthetic BiologyTagsExpressionMammalianMutation25 repeats of BoxB sequencePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-PmCDA1
Plasmid#60818Purposeto overexpress lamprey deaminase in yeastDepositorInsertCDA1
UseTagsFLAGExpressionYeastMutationPromoterGAL10AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
UseTagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pSECC
Plasmid#60820Purpose3rd generation vector. Expresses a sgRNA of interest, Cas9 and CreDepositorInsertsCas9
Cre
UseCRISPR, Cre/Lox, Lentiviral, and Mouse TargetingTagsFlagExpressionMammalianMutationBsmBI site eliminated by C->A; E308GPromoterEFS and EFS (after Cas9-2A)AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorInsertRAD30 (RAD30 Budding Yeast)
UseTagsc-mycExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA
Plasmid#60824Purposeto purify human ITPA from bacteriaDepositorInsertITPA (ITPA Human)
UseTagsHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA_P32T
Plasmid#60825Purposeto purify human ITPA from bacteriaDepositorInsertITPA (ITPA Human)
UseTagsHisExpressionBacterialMutationP32TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSCV-AML1/ETO-IRES-GFP
Plasmid#60832PurposeAML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFPDepositorUseRetroviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NaChBac pTracer CMV2
Plasmid#60835PurposeMammalian expression plasmid containing NaChBac gene with an EGFP reporterDepositorInsertNaChBac
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTargeT gE
Plasmid#60845PurposeExpression of the varicella zoster glycoprotein (gE) in mammalian cellsDepositorInsertORF68 (ORF68 )
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-loxP-hNANOG
Plasmid#60849PurposeLentiviral DOX-inducible expression vector for human NANOGDepositorInserthNANOG (NANOG Human)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 8641 - 8660 of 145688 results