pESC-LEU-PmCDA1
(Plasmid
#60818)
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Purposeto overexpress lamprey deaminase in yeast
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepESC-LEU
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDA1
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Alt namePmCDA.21 cytosine deaminase
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Speciessea lamprey (Petromyzon marinus)
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GenBank IDEF094822
- Promoter GAL10
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer Gal10Pro_F_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Masayuki Hirano (Emory University) and Igor Rogozin (NCBI) contributed to the creation of this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pESC-LEU-PmCDA1 was a gift from Youri Pavlov (Addgene plasmid # 60818 ; http://n2t.net/addgene:60818 ; RRID:Addgene_60818) -
For your References section:
Genome-wide mutation avalanches induced in diploid yeast cells by a base analog or an APOBEC deaminase. Lada AG, Stepchenkova EI, Waisertreiger IS, Noskov VN, Dhar A, Eudy JD, Boissy RJ, Hirano M, Rogozin IB, Pavlov YI. PLoS Genet. 2013;9(9):e1003736. doi: 10.1371/journal.pgen.1003736. Epub 2013 Sep 5. PGENETICS-D-13-01361 [pii] PubMed 24039593
