pET15b-ITPA_P32T
(Plasmid
#60825)
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Purposeto purify human ITPA from bacteria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-15b
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Backbone manufacturerinosine triphosphate pyrophosphohydrolase
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameITPA
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Alt nameinosine triphosphate pyrophosphohydrolase
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SpeciesH. sapiens (human)
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MutationP32T
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Entrez GeneITPA (a.k.a. C20orf37, DEE35, HLC14-06-P, ITPase, My049, NTPase, dJ794I6.3)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-ITPA_P32T was a gift from Youri Pavlov (Addgene plasmid # 60825 ; http://n2t.net/addgene:60825 ; RRID:Addgene_60825) -
For your References section:
Functional study of the P32T ITPA variant associated with drug sensitivity in humans. Stepchenkova EI, Tarakhovskaya ER, Spitler K, Frahm C, Menezes MR, Simone PD, Kolar C, Marky LA, Borgstahl GE, Pavlov YI. J Mol Biol. 2009 Sep 25;392(3):602-13. doi: 10.1016/j.jmb.2009.07.051. Epub 2009 Jul 23. 10.1016/j.jmb.2009.07.051 PubMed 19631656