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Showing: 841 - 860 of 14475 results

  4. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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  5. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

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  7. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  16. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

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  17. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

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  18. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

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  19. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

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  20. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

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Showing: 841 - 860 of 14475 results