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Plasmid#118095PurposeFluorescent reporter for AP-1 activationDepositorInsertmCherry
UseRetroviralTagsExpressionMutationPromoterminimal promoter with AP-1 responsive elementAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR
Plasmid#111177PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_EGFPligand
Plasmid#79129PurposeLentiviral vector for surface expressed EGFP (SFFV promoter)DepositorInsertEGFP surface ligand
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP3535
Plasmid#46886PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseTagsExpressionInsectMutationPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mGSDME
Plasmid#154877PurposeExpresses mGSDME in mammalian cellsDepositorInsertGSDME (Gsdme Mouse)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-BSR-GFP11
Plasmid#68716PurposeSplit GFP assayDepositorInsertbsr
UseRetroviralTagsFLAG and GFP11ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/deltavpx
Plasmid#115807PurposeSFFV promoter expresses gag-gfp fusion with no ORF after CypA promoterDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial sizeInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
UseTagsHis14 and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eCFP
Plasmid#118094PurposeFlourescent reporter for NF-kB activityDepositorInserteCFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CR4
Plasmid#62655PurposeCas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetRDepositorInsertCas9 nuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiFLAGscFv-TM
Plasmid#198303PurposeDisplays anti-FLAG scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiFLAGscFv-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SV40 LT
Plasmid#13970DepositorInsertSV40 LT (SV40gp6 )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-pur H-2Kb
Plasmid#111623PurposeSleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I alleleDepositorInsertH2Kb
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-Climp63
Plasmid#136293Purposeexpression of Climp63DepositorInsertCytoskeletal-linking protein 63 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-Cas9
Plasmid#96924Purposefor Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111DepositorInsertCas9
UseLentiviralTags3xFlag and V5ExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6A-EGFR WT
Plasmid#42665DepositorInsertEGFR (EGFR Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
UseTagsExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3HA-ERHBD-Hoxb8
Plasmid#212932PurposeExpresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell linesDepositorUseRetroviralTagsTriple HA tagExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAVU6+27-F30-2xdBroccoli
Plasmid#66842PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTagsExpressionMammalianMutationPromoterU6Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Neo(+) hSUMF1
Plasmid#24386DepositorInserthSUMF1 (SUMF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-N1
Plasmid#54737PurposeLocalization: N1 Cloning Vector, Excitation: 485, Emission: 507DepositorTypeEmpty backboneUseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLminP_Luc2P_RE27
Plasmid#90369PurposeType II Interferon - STAT1/STAT1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT1Available sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBL101-RUBY
Plasmid#199723PurposeExpresses betalain biosynthesis genes under CaMV 35S promoterDepositorInsert35S-RUBY
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-pA-Hia5
Plasmid#174372PurposePlasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein A - hia5
UseTags6xHis and Protein AExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas3cRh
Plasmid#133773PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXR001: EF1a-CasRx-2A-EGFP
Plasmid#109049PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targetingDepositorInsertCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationPromoterEF1AAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LcV2-Hygro
Plasmid#91977PurposeLentiCRISPR v2 with Hygro cloned in place of PuroDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
PPRE X3-TK-luc
Plasmid#1015PurposePPAR reporter. 3xDR1 sites upstream of a luciferase reporter.DepositorInsertPPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only