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mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-EF1a-mTagBFP2

Plasmid

#160507

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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SABgeolox2DTA

Plasmid

#13447

Depositor

Philippe Soriano

Insert

SABgeolox2DTA

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pXRM15

Plasmid

#63150

Purpose

Met15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

Depositor

Marc Gartenberg

Article

Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

Plasmid

#114433

Purpose

Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6s, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLVTH-siGFP

Plasmid

#12248

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against GFP

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

NGFRdel is in place of GFP on the backbone.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-lox-GFP shRNA p19-2

Plasmid

#14091

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
V1664 pLPS-3'RFP-mito - Acceptor

Plasmid

#11702

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP-mito

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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Thy1-Brainbow-1.0 L

Plasmid

#18725

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; EYFP ; mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pSicoR-shS6K1/2

Plasmid

#81088

Purpose

expresses an shRNA targeting both S6K1 and S6K2 (mouse)

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

S6K1 & S6K2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pROSA26-FlpERT2

Plasmid

#149438

Purpose

ROSA26 targetting vector with tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pVIPR2-ERT2CreERT2pA

Plasmid

#163628

Purpose

Heart-specific tamoxifen-dependent Cre expression

Depositor

Daekee Lee

Article

Chin et al Lab Anim Res. 2020 Sep 15;36:31. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Tags

ERT2 on both N-terminal and C-terminal of Cre

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PL-452 N-EGFP

Plasmid

#19173

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

EGFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRKO

Plasmid

#49456

Purpose

loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

Depositor

Marc Gartenberg

Article

Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

Insert

loxP-kanMX-loxP cassette

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

Plasmid

#137143

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

H134R

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Lox-stop-Lox p53 R270H

Plasmid

#14853

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R270H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Contact mutant R270H. (Murine p53 codon 270 corre…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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