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Addgene
Showing: 8561 - 8580 of 13976 results

  1. pGBK CUP1 Nxf1B

    Plasmid
    #170266
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1B
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    nuclear export factor 1, B6 allele
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Availability
    Academic Institutions and Nonprofits only
    170266-plasmid-map-sequence-id-335102

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  2. pGBK CUP1 Nxf1C

    Plasmid
    #170267
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1C
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    nuclear export factor 1, CAST allele
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Availability
    Academic Institutions and Nonprofits only
    170267-plasmid-map-sequence-id-335103

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  3. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Availability
    Academic Institutions and Nonprofits only
    184064-plasmid-map-sequence-id-365948

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  5. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid
    #203652
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire-IRES-H2B-mCherry
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203652-plasmid-map-sequence-id-402483

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  6. Cas9-T2A-H2B mCitirine

    Plasmid
    #210471
    Purpose
    Coexpression of Cas9 and H2B mCitrine
    Depositor
    Amro Hamdoun
    Insert
    Cas9-T2A-H2B mCitirine
    Use
    CRISPR and Synthetic Biology
    Tags
    mCitrine on C terminal of H2B
    Expression
    Mammalian
    Mutation
    Cas9 contains 3xFLAG tag and nuclear localization…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    210471-plasmid-map-sequence-id-416284

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  15. L3MBTL1 (3P8H)

    Plasmid
    #28265
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 200-530.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mPA-GFP-LaminB1-10

    Plasmid
    #57141
    Purpose
    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. 3HX4

    Plasmid
    #60137
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TgCDPK4:MAC03F-A01:C208088
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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Showing: 8561 - 8580 of 13976 results