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Plasmid#130960PurposeEngineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG
Plasmid#130690PurposeExpresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, G1720_Y1846delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG
Plasmid#130685PurposeExpresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.41-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_Q1684delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130682PurposeExpresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG
Plasmid#130687PurposeExpresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_E1992delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
S1_AscI_V5-gateway_Bsu36I
Plasmid#128467Purposedestination vector with N-terminal V5 tagDepositorInsertdestination vector with N-terminal V5 tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S2_AscI_HA-gateway_Bsu36I
Plasmid#128468Purposedestination vector with N-terminal HA tagDepositorInsertdestination vector with N-terminal HA tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGS-BacA-21222-FLP-3xFLAG
Plasmid#109165PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFlp
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mdm1_pJet1.2
Plasmid#121977Purposefor generating mdm1 riboprobes for WISH in zebrafishDepositorInsertmdm1 (mdm1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 uvrA
Plasmid#112892PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertuvrA-like protein
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3
Plasmid#107301PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP and TALE
UseCRISPRTagsFKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-YJR129c
Plasmid#85118Purposebacterial expression of YJR129cDepositorInsertEFM3 (EFM3 Budding Yeast)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
R2Ct (1-520)
Plasmid#115649Purposeexpression of truncated Rag2 including the C-terminal regionDepositorInsertRag2 (1-520) (Rag2 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag2 (aa 1-520)PromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MAM33MTS (12)
Plasmid#101693PurposeMAM33MTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR I206F (JBEI-3678)
Plasmid#110149PurposeSaGGR site-directed mutation of I206FDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of I206FPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR G91H (JBEI-3677)
Plasmid#110148PurposeSaGGR site-directed mutation of G91HDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of G91HPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only