pSKB3SaGGR I206F (JBEI-3678)
(Plasmid
#110149)
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PurposeSaGGR site-directed mutation of I206F
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSKB3
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGGR
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Alt namegeranylgeranyl reductase
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SpeciesSulfolobus acidocaldarius
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MutationSaGGR site-directed mutation of I206F
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSKB3SaGGR I206F (JBEI-3678) was a gift from Jay Keasling (Addgene plasmid # 110149 ; http://n2t.net/addgene:110149 ; RRID:Addgene_110149) -
For your References section:
Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase. Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD. Structure. 2014 Jul 8;22(7):1028-36. doi: 10.1016/j.str.2014.05.007. Epub 2014 Jun 19. 10.1016/j.str.2014.05.007 PubMed 24954619
