Skip to main content
Addgene
Showing: 8541 - 8560 of 11730 results

  2. pCAG-OSF-PP-ULK1(MIT)2, residues 833-1050

    Plasmid
    #180644
    Purpose
    Mammalian expression vector for ULK1 MIT domains, residues 833-1050. Has Precision protease cleavable N-terminal One-Strep Flag tag. Internal ID: WISP20-37.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    ULK1 (ULK1 Human)
    Use
    Tags
    One-Strep-Flag (OSF)
    Expression
    Mammalian
    Mutation
    Residues 833-1050
    Promoter
    pCAG
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180644-plasmid-map-sequence-id-358523

    Enlarge

  3. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid
    #180641
    Purpose
    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4B (CHMP4B Human)
    Use
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    Residues 156-224
    Promoter
    T7
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180641-plasmid-map-sequence-id-358633

    Enlarge

  6. pBactin-Nedd1-mOrange2-rCM1

    Plasmid
    #196861
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196861-plasmid-map-sequence-id-391830

    Enlarge

  7. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

    Enlarge

  9. PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

    Plasmid
    #187274
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Anillin A740D, E758K
    Promoter
    U6, 5'LTR
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187274-plasmid-map-sequence-id-373247

    Enlarge

  19. pAAV-Syn-FLEX-rc [ArchT-tdTomato]

    Plasmid
    #123607
    Purpose
    AAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Insert
    ArchT-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Syn
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123607-plasmid-map-sequence-id-243930

    Enlarge

  20. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91734-plasmid-map-sequence-id-194569

    Enlarge

Showing: 8541 - 8560 of 11730 results