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Showing: 8501 - 8520 of 54234 results

  1. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

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  5. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Tags
    HA and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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  7. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  8. pTripZ EV

    Plasmid
    #209071
    Purpose
    DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209071-plasmid-map-sequence-id-412293

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  9. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

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  12. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

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  13. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

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  14. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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  20. pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag

    Plasmid
    #179977
    Purpose
    Mammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).
    Depositor
    Konstantin Sparrer
    Article
    Hayn et al Cell Rep. 2021 May 18;35(7):109126.
    Insert
    SARS-CoV-2 E (E Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179977-plasmid-map-sequence-id-353866

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Showing: 8501 - 8520 of 54234 results