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pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Use

Tags

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDV06.2c_DART-VADAR_iRFP720-sensor

Plasmid

#200410

Purpose

pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor detecting iRFP720

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200403

Purpose

Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Article

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mutation

D10A and H840A

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200402

Purpose

Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_18xTEAD_luc2

Plasmid

#173003

Purpose

firefly luciferase reporter vector with 18x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

18xTEAD-luc2

Use

AAV and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124428

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-mMLH1dn

Plasmid

#174825

Purpose

Mammalian expression of mouse MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (codon optimized)

Use

Tags

Expression

Mammalian

Mutation

deletion of residues 758-760

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-mRxxFP1(AfeI)-iP-A

Plasmid

#204625

Purpose

Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Use

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Mutation

Promoter

ponA

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1 kb upstream of IFNB1 CDS

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36880

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

tTA2
tdT-3Myc
insulator

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_FLAG-YAP1

Plasmid

#173001

Purpose

constitutive expression of FLAG-tagged YAP1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

FLAG-YAP1 (YAP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP-5SA

Plasmid

#33093

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-GFP

Plasmid

#190231

Purpose

AAV vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:LIMD1

Plasmid

#108230

Purpose

Lentiviral vector for Tet-inducible LIMD1 expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tet/ON Minimal CMV promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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