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PKG I alpha CAT

Plasmid

#16392

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, S65D

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

Peter Parker, David Bryant

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Use

Tags

Expression

Mammalian

Mutation

D463N

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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PKG I alpha dNPS

Plasmid

#16391

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: delet…

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
CMV-PKCg-mEGFP-N1

Plasmid

#112270

Purpose

IDOCKSgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pMTH PKC gamma

Plasmid

#8412

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pLTR PKC gamma

Plasmid

#8418

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Protein Kinase C gamma (Prkcg Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
mVenus-PKCB1 K371R

Plasmid

#195230

Purpose

Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB1 (PRKCB Human)

Use

Retroviral

Tags

Expression

Mutation

K371R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-pcDNA3-PKCgamma-cys1Acys1B

Plasmid

#21190

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-cys1Acys1B (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Use

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AMPK-β2-FLAG

Plasmid

#40603

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
AMPK-β2-MYC

Plasmid

#40606

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-AMPK-β2

Plasmid

#40604

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-β2 (Prkab2, Prkab2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pMRXIP-GFP-PKD C1ab

Plasmid

#221755

Purpose

Stable expression of GFP-PKD C1ab in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Prkd1(138-343) (Prkd1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 138-343

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex PKC - Peptide 8 GFP RFP

Plasmid

#83564

Purpose

Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG purification tag, TEV Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex PKC - Peptide 8 mCit mCer

Plasmid

#83563

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PRKCA (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG purification tag, TEV Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiex PKC - Peptide 14 mCit mCer

Plasmid

#83565

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge