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Addgene

pBiex PKC - Peptide 8 GFP RFP
(Plasmid #83564)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83564 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBiex-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5259
  • Total vector size (bp) 8106
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC alpha
  • Alt name
    PRKCA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2868
  • Mutation
    Only catalytic domain of PKC (aa 335-672)
  • Entrez Gene
    PRKCA (a.k.a. AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA)
  • Promoter T7 Promoter
  • Tags / Fusion Proteins
    • eGFP
    • mCherry
    • FLAG purification tag (C terminal on insert)
    • 10 nm ER/K Helix
    • TEV Protease Site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Foward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBiex PKC - Peptide 8 GFP RFP was a gift from Sivaraj Sivaramakrishnan (Addgene plasmid # 83564 ; http://n2t.net/addgene:83564 ; RRID:Addgene_83564)
  • For your References section:

    Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency. Sommese RF, Sivaramakrishnan S. J Biol Chem. 2016 Aug 23. pii: jbc.M116.737601. 10.1074/jbc.M116.737601 PubMed 27555323