-
Plasmid#46471DepositorInsertPLCg1(NC) (PLCG1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 542-759PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)
Plasmid#177850PurposeBacterial Expression of AMPKDepositorInsertAMPK (a1b2g1)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-KP1
Plasmid#206504PurposeAAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoterDepositorInsertKP1 capsid
UseAAVTagsExpressionMutationPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo
Plasmid#191397PurposesgRNA targeting alpha-satellites on human chromosome 9DepositorInsertsgRNA targeting alpha satellites on human chromosome 9
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tagged-IRES
Plasmid#35570DepositorInsertLymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
UseLuciferaseTagsFirefly Luciferase and MS2 Stem LoopExpressionMammalianMutationPromoterSV40Available sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YN
Plasmid#74723PurposeExpress C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEntry_SARS-CoV-2-nsp6
Plasmid#168869PurposeGateway-compatible Entry vectorDepositorInsertSARS-CoV-2-nsp6 (ORF1ab )
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9
Plasmid#140237PurposeLentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterEF-1a coreAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA
Plasmid#176745PurposeExpresses Glt-1 fused with BioID2 at the C-terminus in astrocytesDepositorInsertGlt1-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
dxCas(3.7)-VPR
Plasmid#108383PurposeMammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminusDepositorInsertdxCas(3.7)-VPR
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GIT1
Plasmid#15226DepositorInsertG protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
UseTagsGFPExpressionMammalianMutationL425V, E476K and L583PPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Chk1-Flag
Plasmid#22894DepositorInsertChk1 (CHEK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB1679 CMV-HIVNES-GS-Cas13bt3
Plasmid#176317PurposeCMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)DepositorInserthuman codon optimized Cas13bt3
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOmniBac zz TEV YBBR POT1
Plasmid#185444PurposeExpresses human POT1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cellsDepositorInsertPOT1 (POT1 Human)
UseTagsYBBR and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1678 CMV-HIVNES-GS-Cas13bt1
Plasmid#176316PurposeCMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)DepositorInserthuman codon optimized Cas13bt1
UseCRISPRTagsHIV NESExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS1-V5 puro
Plasmid#158641PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS1.DepositorInsertOAS1 (OAS1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only