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Plasmid#192117PurposeLentiviral deliviery of ON VIPER CAR (anti-CD19)-final designDepositorInsertpHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135A
Plasmid#69277PurposeGolden Gate entry vector; 5th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136A
Plasmid#69278PurposeGolden Gate entry vector; 6th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSF280
Plasmid#167675PurposeIntermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-LSSmOrange
Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163062PurposeEntry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseGateway entry vectorTagsmOrange2ExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCl-MCS_miniCMV_TagBFP
Plasmid#134986PurposeThe plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vectorDepositorInsertTagBFP
UseLentiviralTagsExpressionMutationPromoterminCMVAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP670nano
Plasmid#127429PurposeHuman alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertmiRFP670nano-human alpha-tubulin
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x7
Plasmid#58777PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_b4g-HaloTag9
Plasmid#175546PurposeExpression of HaloTag9 at the at the Gogli apparatus of the mammalian cellsDepositorInsertBeta4Gal-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE391
Plasmid#149281PurposeT-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA_FT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU53
Plasmid#167679PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPDK3959
Plasmid#199246PurposeModified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 geneDepositorInsertModified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationPromoterPEBVAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9v2
Plasmid#103140PurposeTetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready siteDepositorInsertdCas9-VPR
UseTagsExpressionYeastMutationRemoved scaffold gRNA from original plasmidPromoterAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMulti-sgRNA-LacZ-DsRed
Plasmid#99914PurposeReceptor plasmid for the assembly of multiple sgRNAs with DsRed coexpressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-boxB-MS2-PP7
Plasmid#75397PurposesgRNA1-boxB-MS2-PP7DepositorInsertsgRNA1-boxB-MS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU54
Plasmid#167680PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1_STf
Plasmid#182006PurposeCMV overexpression of SNAP-tag fast in the lysosome of mammalian cell linesDepositorInsertLAMP1_STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163075PurposeEntry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only