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Plasmid#224529PurposeA Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyADepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nontarget S. pyogenes scaffold CARGO array
Plasmid#191319PurposeContains an array of nontargeting guide RNAsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-mTagBFP2-P2A-mScarlet
Plasmid#196115Purposezebrafish expression of nbt-mTagBFP2-P2A-mScarletDepositorInsertnbt-mTagBFP2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO2-IRES-mScarletI
Plasmid#136896PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.DepositorInsertGFPGO2-IRES-mScarletI
UseLentiviralTags2xNLS on GFP, 3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet
Plasmid#130708PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
iF53 PB-Zim3-dCas9-mScarlet-Hygro
Plasmid#204726PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-oScarlet
Plasmid#137136PurposeIntersectional viral expression of oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225245PurposeMammalian expression of iPASnFR-H in the nucleusDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iPASnFR-H-mScarlet3-3xFLAG-Rab5
Plasmid#225243PurposeMammalian expression of iPASnFR-H fused to Rab5DepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhGC wt T2A tDimer
Plasmid#101720Purposehumanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the mDepositorInsertsCatRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhSyn1 and ribosomal skip sequence T2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG WT
Plasmid#163979PurposeMammalian expression vector encoding human NLRP1 UPA-CARD with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-mScarlet (MNW002)
Plasmid#174137PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarletDepositorInserthuman codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-mScarlet-I-CD4-bla
Plasmid#189980PurposeDonor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertmScarlet-I
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mScarlet-I-CD4-bla
Plasmid#179449PurposeDonor vector to knock in mScarlet-I C-terminal to human PER2 geneDepositorInsertmScarletI
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
Plasmid#196196Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
UseHomology repair donorTagsV5-APEX2-mScarlet-IExpressionMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
RhoA sensor (mScarlet-I_10_LSS-SGFP2_10)
Plasmid#112952PurposeRhoA FRET biosensorDepositorInsertRhoA sensor
UseTagsLSS-SGFP2 and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-WPRE
Plasmid#130990PurposeAAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsmScarletExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt-Electra1-P2A-mScarlet
Plasmid#196113Purposezebrafish expression of nbt-Electra1-P2A-mScarletDepositorInsertnbt-Electra1-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1
Plasmid#124650PurposeSoma-localized C1V1(t/t) for dense expression in excitatory neuronsDepositorHas ServiceAAV9InsertC1V1
UseAAVTagsmScarletExpressionMammalianMutationPromoterCamKIIaAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]
Plasmid#200339PurposemScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001536, flp-17Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only