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Plasmid#220565PurposeLentiviral expression of human EWSR1::WT1(-KTS)DepositorUseLentiviralTagsHAExpressionMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215001PurposeShuttling vector for a mitophagy reporterDepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215002PurposeShuttling vector for an ERphagy reporterDepositorInsertKDEL
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215004PurposeShuttling vector for an autophagy (LC3) reporterDepositorInsertMAP1LC3B (MAP1LC3B Rat)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7
Plasmid#221555PurposeExpresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-3xHA
Plasmid#214986PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tagDepositorTypeEmpty backboneUseTags3xHAExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-4420-mCherry
Plasmid#218828PurposeYeast surface display of an anti-fluorescene scFv and intracellular expression of mCherryDepositorInsert4-4-20 scFv
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-pT231 scFv-GFP
Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PML:1-781
Plasmid#211090PurposeMammalian expression of full-length human PML isoform 13. C-terminal NanoLuc luciferase tag.DepositorInsertPML:M1-L781 (PML Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 13 . E224D annotated sequence conflict. …PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA303.DA9.1B.mRFP
Plasmid#206802PurposemRFP with synthetic promoter HP3 (from Figure S21)DepositorInsertmRFP
UseTagsExpressionMutationPromoterDA9.1B (HP3)Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
GluR2 FL/pRK5
Plasmid#196948PurposePlasmid expressing an antigen targeting the GluR2/GluA2 AMPA receptor subunitDepositorInsertGluR2 (FL) (Gria2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Halo-10-PFN1-G118V
Plasmid#204225PurposeExpression of Halo-Profilin-1(G118V) in tissue culture cellsDepositorInsertPFN1 (PFN1 Human)
UseTagsHalo, TEV site, SGLRSRAQAS linkerExpressionMammalianMutationG118VPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest Lenti CA-GFP
Plasmid#197459PurposeBicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationS65TPromoterCMV, IRESAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-CEBPD
Plasmid#192897PurposeBarcoded piggybac transposon vector with Dox-inducible expression of CEBPDDepositorInsertCEBPD (CEBPD Human)
UseTagsExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AV0-EF1-N-cG
Plasmid#192888PurposeExpression of Nanoluc-P2A-copGFPDepositorInsertNanoluc-P2A-copGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
APT3-VAMP7
Plasmid#168002Purposepositive selection PACE Accessory PlasmidDepositorInsertVAMP7-T3'PAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB19a-GFP
Plasmid#126131PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGE670
Plasmid#153236PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SFXN2
Plasmid#132256PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSFXN2 (SFXN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SFXN5
Plasmid#132292PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSFXN5 (SFXN5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbB15a-GFP
Plasmid#126130PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB18a-GFP
Plasmid#126133PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23117Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB20a-GFP
Plasmid#126132PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterS23200Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB13a-GFP
Plasmid#126116PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23102Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB10a-GFP
Plasmid#126113PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23116Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160A-siR
Plasmid#73975PurposeHDR template to generate Cdk2-T160A-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Ala and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_wt p53
Plasmid#22945Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tagDepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Positron
Plasmid#129253PurposeExpress Positron in mammalian cellsDepositorInsertPositron
UseTagsExpressionMammalianMutationD92N, E199VPromoterCAGAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-BSD (BRD4)
Plasmid#91795PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralTagsExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only