Addgene

Showing: 801 - 820 of 18489 results

Results per page:

Sort results by:

pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
EB3-tdTomato

Plasmid

#50708

Purpose

expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

Depositor

Erik Dent

Article

Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

Insert

EB3 (MAPRE3 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Mutation

Promoter

T5-LacO

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
coel::RFP

Plasmid

#8938

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Available since

Nov. 21, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Lck

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2-epegRNA-Dnmt1

Plasmid

#211372

Purpose

Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MS2-epegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX313-TP53-WT

Plasmid

#118014

Purpose

Constitutive expression of wild-type human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNS2

Plasmid

#64968

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge