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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Robert Malinow, Andres Barria

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDGM6

Plasmid

#110660

Purpose

Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

Depositor

David Russell

Article

Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

Insert

AAV6 cap genes, AAV2 rep genes, adenovirus helper genes

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Use

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Use

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pC0042-PguCas13b crRNA backbone

Plasmid

#103853

Purpose

For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-PguCas13b DR-BbsI-BbsI-polyT

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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VSV.G

Plasmid

#14888

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

VSV-G Envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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SpdCas9 VPR

Plasmid

#188509

Purpose

Expresses FLAG tagged SpdCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Jim Wells, Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV5

Inserts

Caspase 3 (CASP3 Human, Synthetic)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Tags

Expression

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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