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Plasmid#208645PurposeRemove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparation for knock-in of…TagsTwin StrepExpressionMutationTwo SNP (rs749555789, rs760194863) were introduce…PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-FR-2Strep
Plasmid#210312PurposeExpresses the C terminal of SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1pd-HIS
Plasmid#206036PurposeBacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoterDepositorInserthXRCC1
UseTagsHISExpressionBacterialMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-EGFP
Plasmid#210318PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 N-2Strep
Plasmid#210376PurposeExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cellsDepositorInsertSARS-COV-2 N (N SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2T_eGFP
Plasmid#208883PurposeExpress eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations L191T and A225TPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2E_eGFP
Plasmid#208884PurposeExpress eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations K192E and R233EPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
REEP6-2Strep
Plasmid#210351PurposeExpresses REEP6 fused with 2Strep in mammalian cellsDepositorInsertREEP6 (REEP6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-DsRed/eGFP (pXK-442)
Plasmid#208827PurposeThe SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEditC
Plasmid#207532PurposePlasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEditA-RY
Plasmid#207533PurposePlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEditC-RY
Plasmid#207534PurposePlasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS44i8GH-2
Plasmid#207526PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NES 54kDa
Plasmid#201375PurposeNucleocytoplasmic transport probeDepositorInsertMAPK_NES-EGFP-4PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209016PurposeOverexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209011PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mt deltaN20 TFB2MdeltaC7
Plasmid#205060PurposeTFB2M protein expressionDepositorInsertTFB2M (TFB2M Human)
UseTagsExpressionBacterialMutationLacks N-terminal 20 aa and C-terminal 7 aaPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10D(E215Q)-HA
Plasmid#209248PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseTagsHAExpressionMammalianMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D(E215Q)-HA
Plasmid#209260PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseRetroviralTagsHAExpressionMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only