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Plasmid#125169PurposeExpresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertMTF2 (MTF2 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-INPP4BC842A-CAAX
Plasmid#116858Purposeinactive plasma membrane targetted PI(3,4)P2 phosphataseDepositorInsertINPP4B (INPP4B Human)
UseTagsHRAS(172-189) and TagBFP2ExpressionMammalianMutationcatalytic cysteine 842 changed to alaninePromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
902 pLNCX myr HA Akt1 K179M
Plasmid#9006DepositorInsertAkt1 K179M (AKT1 Human)
UseRetroviralTagsHA and myrExpressionMammalianMutationK179M. kinase dead.PromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-V5 IRES-puro
Plasmid#107059PurposeExpression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationK785RPromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of RGSā¦ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-termā¦PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorInsertNbVHH05-HA-His (UBE2J1 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/20]
Plasmid#206744PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SpyCatcher-ELP-GFP
Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
UseTags6x His Tag, GFP, and TEV TagExpressionBacterialMutationPromoterT5 promoter/lac operator elementAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
W118-1_Flag-hIRAK1
Plasmid#180405Purposelentiviral transduction of human IRAK1 (with an N-terminal Flag tag) geneDepositorInsertIRAK1 (IRAK1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST scFv [N100/13]
Plasmid#190521PurposeMammalian Expression of GST scFV. Derived from hybridoma N100/13.DepositorInsertGST (Schistosoma japonicum) recombinant scFV
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flrt2-Fc-His
Plasmid#72074PurposeExpresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertFlrt2 (Flrt2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1 C121S
Plasmid#53197PurposeExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationC121SPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-GFP
Plasmid#171571PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cellsDepositorInsertNb127D01-GFP (CXCR2 Human)
UseTagsGFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorInsertMBP-MEK1 S218TAG (MAP2K1 Synthetic, Human)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/44]
Plasmid#206743PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-Myc3-HPS4
Plasmid#133931PurposeExpresses 3xMyc-HPS4 construct in mammalian cellsDepositorInsertHPS4 (HPS4 Human)
UseTags3xMyc tagExpressionMammalianMutationGlutamic acid 229 to Glycine; Valine 552 to Methiā¦PromoterCMV I.E.Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK1A-2A-GFP
Plasmid#118270PurposeExpresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only