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Addgene
Showing: 781 - 800 of 822 results

  5. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #108274
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    EF1α promoter (1.1kb short version)
    Availability
    Academic Institutions and Nonprofits only
    108274-plasmid-map-sequence-id-262430

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  12. pJMK004

    Plasmid
    #34616
    Purpose
    Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator
    Depositor
    Adam Cohen
    Article
    Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n
    Insert
    Arch D95N
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Asparagine
    Promoter
    CaMKII
    Availability
    Academic Institutions and Nonprofits only
    34616-plasmid-map-sequence-id-41436

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  15. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

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  17. OPN3-DuET

    Plasmid
    #213357
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPN3 (OPN3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213357-plasmid-map-sequence-id-430879

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Showing: 781 - 800 of 822 results