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Showing: 1951 - 2000 of 15602 results

  4. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  5. pCHC025 (∆CBBp)

    Plasmid
    #226203
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226203-plasmid-map-sequence-id-453509

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  6. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226206-plasmid-map-sequence-id-452437

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  15. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid
    #139457
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139457-plasmid-map-sequence-id-308472

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  16. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid
    #139458
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139458-plasmid-map-sequence-id-308461

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  17. pDEST-EGFP-Cx43ML-N1

    Plasmid
    #49860
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. No s…
    Promoter
    CMV
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNA3.2-Cx43-ML-HA

    Plasmid
    #49857
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. Cont…
    Promoter
    CMV
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3.2-Cx43-NoStart

    Plasmid
    #49853
    Purpose
    Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon mutated to from methionine to isoleuc…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Use
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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  23. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid
    #226205
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226205-plasmid-map-sequence-id-453511

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  26. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid
    #139459
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139459-plasmid-map-sequence-id-308470

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  28. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Depositor
    Carolyn Lee-Parsons, Erin Cram
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123181-plasmid-map-sequence-id-241286

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  29. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. CBSH3- 3m shRNA3

    Plasmid
    #160213
    Purpose
    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    160213-plasmid-map-sequence-id-314924

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  38. CBSH3- 3m shRNA2

    Plasmid
    #160211
    Purpose
    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160211-plasmid-map-sequence-id-313962

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  39. CBSH3- 3m shRNA1

    Plasmid
    #160209
    Purpose
    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Tags
    Expression
    Mutation
    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…
    Promoter
    mouse U6 promoter
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160209-plasmid-map-sequence-id-313960

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  44. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140976-plasmid-map-sequence-id-300659

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  49. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid
    #140977
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoB-Rluc8 (GNAO1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140977-plasmid-map-sequence-id-300660

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Showing: 1951 - 2000 of 15602 results