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mCherry-Calnexin-N-14
Plasmid#55005PurposeLocalization: Endoplasmic Reticulum, Excitation: 587, Emission: 610DepositorInsertCalnexin (CANX Human)
UseTagsmCherryExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-K47Q/R354Q
Plasmid#207323PurposeVSV-G mutant with abolished receptor binding, but maintained fusogenic capacityDepositorInsertVSV-G (VSIVgp4 Vesicular Stomatitis Virus)
UseTagsExpressionMammalianMutationK47Q/R354QPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Oct3/4
Plasmid#13366PurposeRetroviral expression of mouse Oct3/4DepositorInsertPOU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag-Keap1
Plasmid#28023DepositorInsertKeap1 (KEAP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PA-Cre
Plasmid#122960PurposePhotoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)DepositorInsertpcDNA3.1_PA-Cre
UseCre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorInsertPGC-1 alpha promoter (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bx
Plasmid#51552PurposeExpresses Bxb1 integrase in mammalian cells.DepositorInsertBxb1 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL093 pattB_exorh:EGFP_MCS
Plasmid#213514PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag Rac2
Plasmid#12192DepositorInsertRac2 (RAC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsUseTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2attNG-H11-short
Plasmid#51546Purposep2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).DepositorInsertGFP4m
UseHuman targetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKOR1
Plasmid#133446PurposeEscherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selectionDepositorTypeEmpty backboneUseE.coli/s.aureus shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23109
Plasmid#113151PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
UseTagsExpressionBacterialMutationD10A & H840APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-mNeonGreen
Plasmid#137804PurposeVisualization of the Endoplasmic ReticulumDepositorArticleInsertKDEL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag
Plasmid#208051PurposeEmpty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with FlagDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-A3-HA-ubiquitin
Plasmid#220145PurposeExpresses A3-Ub for E3 substrate identification by E-STUBDepositorInsertUbiquitin C (UBC Human)
UseTagsA3-HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorInsertSpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterchicken β-actin promoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE50
Plasmid#90389PurposePPARg/RARa - PPARg gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterDR1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR OVA/p2a/mCherry-CaaX
Plasmid#113030Purposefor lentivirus production of ovalbumin and mcherry-caaxDepositorInsertOVA/p2a/mCherry-CaaX
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorInsertEZH2 (EZH2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only
