10,117 results

1. CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

   Plasmid

   #225753

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONRPEX18Tp-SceI-pheS

   Plasmid

   #111608

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mPlum-N1

   Plasmid

   #54629

   Purpose

   Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Type

   Empty backbone

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAID5.3-C

   Plasmid

   #145813

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Use

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-TetOn-dCas9-KRAB

   Plasmid

   #115545

   Purpose

   Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGE-T7

   Plasmid

   #153021

   Purpose

   Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

   Depositor

   Vito Valiante

   Article

   Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS307

   Plasmid

   #51785

   Purpose

   an integrating (YIp) vector with a LYS2 selectable marker

   Depositor

   David Stillman

   Article

   Eriksson et al Biotechniques. 2004 Feb;36(2):212-3.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

   Plasmid

   #71485

   Purpose

   Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

    Plasmid

    #175572

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCerulean3-C1

    Plasmid

    #54605

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9 plasmid

    Plasmid

    #100091

    Purpose

    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFlag-NLS-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSL

    Plasmid

    #51493

    Purpose

    T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pR26-CMVconst

    Plasmid

    #127373

    Purpose

    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV enhancer/promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-6xGLY-3xFLAG-kanMX6

    Plasmid

    #20754

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNBU2_repA1

    Plasmid

    #211910

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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