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Showing: 61 - 80 of 15679 results

  2. pQ2aB

    Plasmid
    #124887
    Purpose
    Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP
    Depositor
    Nevil Singh
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Blue Flourescent Protein, Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    124887-plasmid-map-sequence-id-243838

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  3. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  4. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  5. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  6. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

    Plasmid
    #194925
    Purpose
    mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho
    Depositor
    Hao Xu
    Article
    Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194925-plasmid-map-sequence-id-393400

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  7. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  8. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  9. NK803

    Plasmid
    #166057
    Purpose
    Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta
    Depositor
    Nicole King
    Article
    Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:
    Inserts
    Pac puromycin resistance gene
    LifeAct-mCherry
    Use
    S. rosetta expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166057-plasmid-map-sequence-id-330645

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  11. pHR_SFFV*/Puro2a-PAG1-GFP

    Plasmid
    #198223
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Lentiviral
    Tags
    Followed by EGFP, after a flexible linker and Pre…
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198223-plasmid-map-sequence-id-389384

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  12. pCWX-R4DESTR2-PGK-PURO

    Plasmid
    #114319
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Puromycin resistance
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    114319-plasmid-map-sequence-id-220088

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  13. pAK_DR30_CasRx_Puro

    Plasmid
    #134845
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    134845-plasmid-map-sequence-id-266706

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  14. pGMC00021 (aka pRC0432)

    Plasmid
    #195305
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with puromycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195305-plasmid-map-sequence-id-385584

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  17. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  18. pRosettav2

    Plasmid
    #115557
    Purpose
    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments
    Depositor
    John Doench
    Insert
    Hygromycin-Blasticidin-Puromycin-EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115557-plasmid-map-sequence-id-226246

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  19. psp-161-si1(sigfp)

    Plasmid
    #58801
    Purpose
    is a lentiviral vector coding the shRNA si1 (sigfp) and the gene of resistance to puromycin.
    Depositor
    Branka Horvat
    Insert
    si1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Human H1
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58801-plasmid-map-sequence-id-91096

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  20. pGMC00017 (aka pRC0162)

    Plasmid
    #195319
    Purpose
    Lentiviral guide RNA backbone derived from pSB700 with puromycin
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195319-plasmid-map-sequence-id-387464

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Showing: 61 - 80 of 15679 results