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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAND_Reporter

Plasmid

#60591

Purpose

AND gate reporter

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_Reporter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_RPA1_RepA-N

Plasmid

#110032

Purpose

Protein expression and purification of RPA1_RepA-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA1_RepA-N (RPA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pReporter_9

Plasmid

#60569

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_9

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_13_switchBP

Plasmid

#62848

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_13_switchBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_SA-2x

Plasmid

#79369

Purpose

Assays activity of transcriptional activators fused to SA Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N10_trigger

Plasmid

#132728

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N10_switch

Plasmid

#132724

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pReporter_11

Plasmid

#60571

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_11

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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37 pCOLI_K_STREP

Plasmid

#160263

Purpose

N-term 2xStrepII 3C cleavable Kan resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Use

Tags

2xStrepII

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pReporter_10

Plasmid

#60570

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_10

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ_3WJrep_N11_trigger

Plasmid

#132733

Purpose

T7 RNAP-driven expression of 3WJ repressor index 11 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N11_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLitTREPitt

Plasmid

#18661

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

TREPitt

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_G102H_ADRB2_LucReporter

Plasmid

#129794

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

G102H

Promoter

N/A

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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Reporter

Plasmid

#36008

Depositor

Larry Gerace

Article

Ohba et al J Cell Biol. 2004 Dec 20;167(6):1051-62.

Insert

LAP2β-TM

Use

Reporter

Tags

11kD FRB and EGFP

Expression

Mammalian

Mutation

FRB is residues 2025-2114 of FRAP, LAP2β-TM is re…

Promoter

CMV

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pREP4xN_ccdB

Plasmid

#79022

Purpose

Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection

Depositor

Göran Akusjärvi, Pernilla Bjerling

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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