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Showing: 1 - 20 of 26 results

  1. Barcoded self-reporting transposon calling card collection

    Plasmid Kit
    #1000000213
    Purpose
    'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.
    Depositor
    Rob Mitra
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  2. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  3. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  5. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems
    Depositor
    Dominic Esposito
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  6. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit
    #1000000115
    Purpose
    B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
    Depositor
    Carol Gross
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit
    #1000000219
    Purpose
    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.
    Depositor
    Alexis Komor
    Available since
    May 1, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. dRMCE Plasmids

    Plasmid Kit
    #1000000015
    Purpose
    Takes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous loci
    Depositor
    Rolf Zeller
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Available since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Used to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.
    Depositor
    Keith Joung
    Available since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. Ubiquiton In Vitro Kit

    Plasmid Kit
    #1000000242
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. CRISPR-Cas9 Gateway System for Physcomitrella patens

    Plasmid Kit
    #1000000151
    Purpose
    CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.
    Depositor
    Magdalena Bezanilla
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. openCIDAR MoClo Toolkit

    Plasmid Kit
    #1000000227
    Purpose
    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.
    Depositor
    Eric Young
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only

  19. CyanoGate Kit

    Plasmid Kit
    #1000000146
    Purpose
    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.
    Depositor
    Alistair McCormick
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  20. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Paul Freemont
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 26 results