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Showing: 1 - 20 of 30052 results

  5. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid
    #225432
    Purpose
    Transcriptional unit of BeYDV Rep/RepA (C1:C2)
    Depositor
    Diego Orzaez
    Insert
    pNOS:Rep/RepA:tNOS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225432-plasmid-map-sequence-id-447739

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  6. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid
    #225433
    Purpose
    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.
    Depositor
    Diego Orzaez
    Insert
    4xCBS:mDFR:Rep/RepA:Tnos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225433-plasmid-map-sequence-id-447737

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  7. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Article
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Tags
    Expression
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

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  8. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

    Plasmid
    #225438
    Purpose
    Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.
    Depositor
    Diego Orzaez
    Insert
    CBS-FRT-OCS-BeYDVRepRepA-tNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225438-plasmid-map-sequence-id-447740

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  10. pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

    Plasmid
    #225437
    Purpose
    Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.
    Depositor
    Diego Orzaez
    Insert
    CBS-att-OCS-BeYDVRep/RepA-tNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225437-plasmid-map-sequence-id-447734

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  14. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  15. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  19. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK1
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

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Showing: 1 - 20 of 30052 results