-
Plasmid#128155PurposeExpression of Sar1 with T39N dominant negative mutationDepositorInsertSar1
UseTagsEYFPExpressionMammalianMutationT39NPromoterAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mScarletI-NLS-blast
Plasmid#136260PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-sTomato-NLS-blast
Plasmid#136259PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-LacI
Plasmid#183921PurposeLac repressor labeled with tdTomato; can be used to label lacO sitesDepositorInserttdTomato-LacI (lacI Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LacI-NLS-VP16
Plasmid#103836PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInsertEGFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-VSVG-RUSH
Plasmid#166907PurposeRUSH cargo: Str-Ii_IRES_VSVG-SBP-HaloDepositorInsertHaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mApple-TNFa-RUSH
Plasmid#166902PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mAppleDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EpsilonCOP EGFP
Plasmid#128151PurposeExpression of epsilon COPDepositorInsertepsilon COP (COPE Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-mOrange2
Plasmid#166899Purposemammalian expression of Sar1b tagged with mOrange2DepositorInsertmOrange2
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
mApple-GPI-RUSH
Plasmid#166904PurposeRUSH cargo: Str-KDEL_IRES_SBP-mApple-GPIDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VPR
Plasmid#183918PurposeVPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VPR
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pLV 4xSTAR-mTFP-NLS-blast
Plasmid#136257PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmTFP-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI
Plasmid#103839PurposeFlourescent marker for lacO arraysDepositorInserttagBFP-LacI
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL R167W-pRc/CMV
Plasmid#22082DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant converting aa 167 from Arginine to T…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-LacI-NLS-VP16
Plasmid#103837PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arraysDepositorInserttagBFP-LacI-NLS-VP16
UseTagsExpressionMammalianMutationPromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag
Plasmid#29497DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI. PGK-puro
Plasmid#136266PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletIExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHMcc(delta16 PHM 356s)
Plasmid#25455DepositorInsertPHM (Pam Rat)
UseTagsExpressionMammalianMutationContains the catalytic core of PHM, including aa …PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato. PGK-puro
Plasmid#136264PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH50
Plasmid#107254PurposeVipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.DepositorInsertvipp1-gfp-fkbp12
UseSuicide vector for destined for double homologous…TagsGFP and FKBP12ExpressionBacterialMutationPromoternativeAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-VHL(1-155)
Plasmid#29500DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsExpressionInsectMutationVHL(1-155)onlyPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec23A
Plasmid#166894Purposemammalian expression of Sec23A tagged with mCherryDepositorInsertSEC23A (SEC23A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-H79G-YFP
Plasmid#166898Purposemammalian expression of Sar1b-H79G tagged with YFPDepositorInsertSar1b-H79G
UseTagsYFPExpressionMammalianMutationH79 to GPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
ptagRFP-MS2coatProtein
Plasmid#103831PurposeLive cell reader for MS2 loop RNA productionDepositorInsertMS2 coat protein (MCP) (cp )
UseTagstagRFPExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MS2coatProtein
Plasmid#103832PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmCherry-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Sec23A
Plasmid#166892Purposemammalian expression of Sec23A tagged with HaloTagDepositorInsertSEC23A (SEC23A Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-Sec31
Plasmid#166895Purposemammalian expression of Sec31 tagged with HaloTagDepositorInsertSec31
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEAK-bKal7
Plasmid#145800Purposeexpresses Kalirin-7 with the b-front in mammalian cellsDepositorInsertbKalirin-7 (Kalrn Rat)
UseTagsExpressionMammalianMutationPromoterEIF2aAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-H79G-mOrange2
Plasmid#166900Purposemammalian expression of Sar1b-H79G tagged with mOrange2DepositorInsertSar1b
UseTagsmOrange2ExpressionMammalianMutationH79GPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-VSVG
Plasmid#11915DepositorInsertVSVG
UseTagsExpressionMammalianMutation(L64F/T65S/V163A/T203H). The PAGFP also contains …PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Sec23A
Plasmid#166893Purposemammalian expression of Sec23A tagged with mEmeraldDepositorInsertSEC23A (SEC23A Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-tdMCP-tdTomato
Plasmid#183938Purposetandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tagDepositorInserttdMCP-tdTomato (cp Synthetic)
UseTagsNLS-HAExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmKate2-MS2coatProtein
Plasmid#103833PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmKate2-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS--GFP-p300
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVenus-VSVG
Plasmid#11914DepositorInsertVSVG
UseTagsExpressionMammalianMutationF46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VP16
Plasmid#183917PurposeVP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VP16 (UL48 Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arf Q71L CFP
Plasmid#128149PurposeExpression of Arf Q71LDepositorInsertArf1 (ARF1 Human)
UseTagsExpressionMammalianMutationQ71LPromoterAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-NLS
Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Venus-GalT
Plasmid#11931DepositorInsertGalT (GALT Human)
UseTagsExpressionMammalianMutationAmino acids 1-60 of the human galactosyltransfera…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TetR-NLS-VP16
Plasmid#103834PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorInsertEGFP-TetR-NLS-VP16 (tetR )
UseTagsExpressionMammalianMutationPromoterSV2Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS-GFP-VP16
Plasmid#183923PurposeVP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-VP16
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only