Skip to main content
Addgene
Showing: 151 - 200 of 314 results

  1. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    Human ferritin
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177425-plasmid-map-sequence-id-347947

    Enlarge

  2. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

    Enlarge

  6. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

    Enlarge

  7. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

    Enlarge

  8. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

    Enlarge

  10. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177429-plasmid-map-sequence-id-348456

    Enlarge

  11. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

    Enlarge

  13. Venus-tBid-4E-pEGFP-C1

    Plasmid
    #166743
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    tBid-4E (Bid Mouse)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166743-plasmid-map-sequence-id-329004

    Enlarge

  20. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

    Enlarge

Showing: 151 - 200 of 314 results