Skip to main content
Addgene
Showing: 121 - 160 of 2389 results

  2. psiT-GEM(0)

    Plasmid
    #220446
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220446-plasmid-map-sequence-id-443761

    Enlarge

  4. psiT-GEM(2)

    Plasmid
    #220448
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220448-plasmid-map-sequence-id-438388

    Enlarge

  5. psiT-GEM(1)

    Plasmid
    #220447
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220447-plasmid-map-sequence-id-436669

    Enlarge

  11. pGEM-T ZfHoxb4a

    Plasmid
    #27523
    Depositor
    Victoria Prince
    Insert
    Hoxb4a (hoxb4a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pGEM-T ZfHoxd10a

    Plasmid
    #27543
    Depositor
    Victoria Prince
    Insert
    Hoxd10a (hoxd10a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. pGEM-T ZfHoxd11a

    Plasmid
    #27544
    Depositor
    Victoria Prince
    Insert
    Hoxd11a (hoxd11a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. pGEMHE-NLS-mEGFP

    Plasmid
    #105527
    Purpose
    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal
    Depositor
    Melina Schuh
    Article
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Insert
    Nucleoplasmin
    Use
    In vitro transcription
    Tags
    mEGFP
    Expression
    Mutation
    NLS of nucleoplasmin (encoding amino acids KRPAAT…
    Promoter
    T7
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    105527-plasmid-map-sequence-id-201844

    Enlarge

  22. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    GEM-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32442-plasmid-map-sequence-id-117095

    Enlarge

  37. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

    Enlarge

  38. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Expression
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

    Enlarge

Showing: 121 - 160 of 2389 results