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Plasmid#220079PurposeSAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-C389S-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationC389SdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA335
Plasmid#216069PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; StopDepositorInsertStop; IRES2_v1; Start; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP
Plasmid#220075Purposeexpresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBPDepositorInsertOSBPL5 H478/9A (OSBPL5 Human)
UseTagsExpressionMammalianMutationH478/9A, deltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaORDdeltaTMD-FKBP
Plasmid#220074Purposeexpresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaORDdeltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaORDdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1071-Lenti-Single loxP
Plasmid#217892PurposeLentiviral vector, entry vector for Lentiviral Switch-OVERDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p223-Pulse-Switch
Plasmid#217889PurposeLentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiRDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ARMS1-ProLink2-MRGPRX3
Plasmid#213962PurposeExpresses MRGPRX3 in mammalian cellsDepositorInsertMRGPRX3 (MRGPRX3 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA294
Plasmid#215968PurposeFragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoterDepositorHas ServiceCloning Grade DNAInsertU1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA018
Plasmid#215976PurposeFragmid fragment: (N' terminus) 4xVP16 transcritional activatorsDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; VP64_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA020
Plasmid#216004PurposeFragmid fragment: (Cas protein) binds PP7 stem loopsDepositorInsertPP7 Core Protein (PCP)_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_162
Plasmid#216092PurposeCas9 [Sp] CRISPRi targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA366
Plasmid#215970PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsert5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA436
Plasmid#216035PurposeFragmid fragment: (Cas protein) binds MS2 stem loopsDepositorInsertMS2_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA440
Plasmid#216036PurposeFragmid fragment: (Cas protein) binds to TRE in absence of doxycyclineDepositorInsertTetR_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA201
Plasmid#216026PurposeFragmid fragment: (Cas protein) firefly luciferaseDepositorInsertFluc_v1.1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA299
Plasmid#216029PurposeFragmid fragment: (Cas protein) NNGG PAMDepositorInsertCas9_v1.1 [Slug]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA296
Plasmid#216028PurposeFragmid fragment: (Cas protein) NNNNCC PAMDepositorInsertNme2Cas9_v1.1 [Nme2]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA184
Plasmid#216047PurposeFragmid fragment: (C' terminus) for prime editing; using PEmaxDepositorInsertSV40NLS_v1.2; MMLV-RT_v1.2; SV40NLS_v1.3; c-mycNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only