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Plasmid#32450DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationK193A (S3V, G96R, and P108L also present)PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.2_mTet3
Plasmid#79625PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM_Puro_shRNA n.1_mTet3
Plasmid#79624PurposeExpression of shRNA for mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseLentiviralTagsnoneExpressionMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ERBB4 (E317K)
Plasmid#29537DepositorInsertERBB4 (ERBB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
p5A1-Ti
Plasmid#92018PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219V, C151APromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta N53
Plasmid#19950DepositorInsertcullin 1 delta N53 (CUL1 Human)
UseTagsHAExpressionMammalianMutationdeletion of 53 residues from the NH2-terminus(de…PromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
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ATF4 19: (SL)RLuc.5’ATF4.GFP
Plasmid#21865DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStem Loop upstream of ATF4PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMM549
Plasmid#112531PurposepZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIhh715bp-Luc
Plasmid#53606PurposeReporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SHIV327C-HuA10-gp160
Plasmid#123270PurposeMammalian expression plasmid for Env from the SHIV327C-Hu A10 strainDepositorInsertSHIV327C-Hu A10 Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Trib2
Plasmid#131155PurposeMammalian expression of flag-tagged mouse TRIB2DepositorInsertTrib2 (Trib2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PYDonly-NLRP11.MYC
Plasmid#131198Purposemammalian expressionDepositorInsertPYD domain of NLRP11 (NLRP11 Human)
UseTagsmycExpressionMammalianMutationcontains only the pyrin domain (amino acids 1-128)PromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
Plasmid#106112PurposeInducible gene expression of FlagHA-PRKRA sgRNA PAM mutantDepositorInsertPRKRA (PRKRA Human)
UseTagsFlag and HAExpressionMammalianMutationsilent mutation of Gly32 (GGG-->GGT)PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-FLAG PLU-HIS
Plasmid#113173PurposeBaculovirus expression of PNG and PLUDepositorInsertPNG, PLU
UseBaculovirusTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 short UTR
Plasmid#21639DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV T274V KSR1 IRES GFP
Plasmid#25977DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged threonine 274 to valinePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV S392A KSR1 IRES GFP
Plasmid#25978DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 392 to alaninePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev
Plasmid#101340PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.DepositorInsertGFP, tat, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only