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Plasmid#50872PurposeExpresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationL671C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)
Plasmid#50871PurposeExpresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationE670C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APP695
Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLT3-D835Y-MSCV-IRES-GFP
Plasmid#184780PurposeExpresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cellsDepositorInsertFLT3 (FLT3 Human)
UseRetroviralTagsExpressionMammalianMutationActivating tyrosine kinase domain mutation (D835Y)PromoterMSCVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Dppa2
Plasmid#40799DepositorInsertDppa2 (Dppa2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal CMV + TetOAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
UseTagsFLAG-HaloTagExpressionMammalianMutationPromoterEndogenous TERT promoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB-1753-Nluc I9A/W10A
Plasmid#158604PurposePlasmid expressing KB-1753-Nluc I9A/W10A in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc L118A
Plasmid#158606PurposePlasmid expressing GRK2(RH)-Nluc L118A in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pWPI-N3ICD
Plasmid#185524PurposeConstitutive overexpression of human NOTCH3 intracellular domainDepositorInsertNOTCH3 intracellular domain (NOTCH3 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-N1ICD
Plasmid#185525PurposeConstitutive overexpression of human NOTCH1 intracellular domainDepositorInsertNOTCH1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LR (hsp70I: cPLA2-mK2)
Plasmid#162564PurposeMeasuring cPLA2 activation in live cells after heat shock.DepositorUseGateway donor vectorTagsmK2ExpressionMutationPromotercmlc2Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4
Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4
Plasmid#62900PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
ML1Nmut_in_pEGFP-C1
Plasmid#67796PurposeMammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFPDepositorInsertML1N*2(7Q)-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBG100-TCPTP
Plasmid#33365DepositorInsertTCPTP-45 (PTPN2 Human)
UseTags6xHIS - cleavableExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs3-TIAM1
Plasmid#86143PurposeRetroviral vector expressing TIAM1-IRES-RFPDepositorInsertTIAM1 (TIAM1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-mSox2
Plasmid#20908PurposepiggyBac vector with tet inducible mSox2DepositorInsertSox2 (Sox2 Mouse)
UsepiggybacTagsIRES-bGeoExpressionMammalianMutationPromoterAvailable sinceApril 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Hd32
Plasmid#163963PurposeExpresses TPMV H glycoprotein in mammalian cellsDepositorInsertTPMV H glycoprotein
UseTagsExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only